abstract：:Neisseria meningitidis, but not Haemophilus influenzae, damage cultured human endothelial cells. We have undertaken a study to generate genetically and structurally defined lipopolysaccharide (LPS) mutant strains of meningococci for functional studies to assess the role of surface exposed oligosaccharides in imparting...

journal_title：Microbial pathogenesis

authors： Jennings MP,Bisercic M,Dunn KL,Virji M,Martin A,Wilks KE,Richards JC,Moxon ER

更新日期：1995-12-01 00:00:00

abstract：:Genital serovariants of Chlamydia trachomatis establish infection by attachment, entry and multiplication within human endometrial epithelial cells. In previous studies, a chlamydial recombinant Escherichia coli was identified which exhibited a specific adherent phenotype to endometrial epithelial cells closely resemb...

journal_title：Microbial pathogenesis

authors： Schmiel DH,Raulston JE,Fox E,Wyrick PB

更新日期：1995-10-01 00:00:00

abstract：:The virulence of Staphylococcus aureus opsonized with an antiserum raised against a recombinant fibronectin-binding protein (FnBP) was compared with homologous, non-opsonized bacteria (treated with pre-immune serum) in a mouse mastitis model. Virulence was evaluated comparing the number of bacteria removed from the in...

journal_title：Microbial pathogenesis

authors： Mamo W,Jonsson P,Müller HP

更新日期：1995-07-01 00:00:00

abstract：:In this study we compared the host response to Listeria monocytogenes in 129 REJ mice with listeria-resistant (C57Bl/6j) and susceptible (Balb/c) mouse strains. In all experiments mice were inoculated by the i.p. route. 129 REJ mice and Balb/c mice were sensitive to listeriosis whilst C57Bl/6j mice were relatively res...

journal_title：Microbial pathogenesis

authors： Gahan CG,Collins JK

更新日期：1995-05-01 00:00:00

abstract：:We obtained biologically active purified leukotoxin (Lkt) from Pasteurella haemolytica serotypel, strain 12296 using preparative sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Three species of Lkt of molecular masses 95, 100, and 104 kDa were obtained. Purity of all three species of Lkt was conf...

journal_title：Microbial pathogenesis

authors： Yoo HS,Rajagopal BS,Maheswaran SK,Ames TR

更新日期：1995-04-01 00:00:00

abstract：:Group A streptococci demonstrate a number of distinct ways to interact with the human fibrinolytic system to acquire unregulatable cell-surface enzymatic activity. Interactions between bacteria, fibrinogen, streptokinase and plasminogen resulted in acquisition of cell-associated enzymatic activity that can lyse fibrin...

journal_title：Microbial pathogenesis

authors： Wang H,Lottenberg R,Boyle MD

更新日期：1995-03-01 00:00:00

abstract：:Candida albicans yeast cells bind soluble human plasma fibronectin (Fn) through a glycoprotein receptor (adhesin) located on the cell surface. This work demonstrates that a 120 kDa proteolytic fragment of Fn encompassing the cell binding domain binds more avidly to the yeast cell adhesin than does the parent Fn molecu...

journal_title：Microbial pathogenesis

authors： Penn C,Klotz SA

更新日期：1994-12-01 00:00:00

abstract：:ADP-ribosylating protein exotoxins from Vibrio cholerae (CT) and Escherichia coli (LT-I) share two short regions of sequence similarity with Bordetella pertussis toxin (PT). Previous studies have indicated that substitution of arginine for lysine 7 within the first region of CT drastically decreases ADP ribosyltransfe...

journal_title：Microbial pathogenesis

authors： Vadheim KL,Singh Y,Keith JM

更新日期：1994-11-01 00:00:00

abstract：:Programmed cell death is part of normal development and homeostasis. Apoptosis induced by bacteria appears to contribute to infectious diseases. Some bacteria produce toxins to kill host cells by the same pathway, apoptosis, through different mechanisms including pore formation, protein synthesis inhibition or adenyla...

journal_title：Microbial pathogenesis

authors： Chen Y,Zychlinsky A

更新日期：1994-10-01 00:00:00

abstract：:The gene gseA, involved in the expression of the genus-specific epitope of chlamydial lipopolysaccharide (LPS), was analyzed by temperature gradient gel electrophoresis (TGGE) to visualize nucleotide sequence variations among the 15 serovars of Chlamydia trachomatis. Sequence analysis showed that the TGGE melting-prof...

journal_title：Microbial pathogenesis

authors： Mamat U,Löbau S,Persson K,Brade H

更新日期：1994-08-01 00:00:00

abstract：:The 90 kb virulence plasmids of Salmonella biosers Gallinarum and Pullorum (expressed as serovar Gallinarum) are non-conjugative. They were, however, found to be readily mobilized by IncFI and IncFIV plasmids, but not by conjugative IncA, IncB = C, IncH, IncL and IncM R plasmids. No virulence plasmids of serovars Chol...

journal_title：Microbial pathogenesis

authors： Ou JT,Lin MY,Chao HL

更新日期：1994-07-01 00:00:00

abstract：:The changes in the cell surface carbohydrates of the eustachian tube (ET) and middle ear subsequent to the intranasal (i.n.) inoculation of Streptococcus pneumoniae (Spn) type 6A were studied in the chinchilla model of otitis media (OM) using a lectin histochemical technique with six different lectins (SNA, WGA, Succ ...

journal_title：Microbial pathogenesis

authors： Linder TE,Daniels RL,Lim DJ,DeMaria TF

更新日期：1994-06-01 00:00:00

abstract：:Two highly passaged laboratory strains of human cytomegalovirus (HCMV), AD169 and Towne, were tested for their ability to infect and replicate in THP-1 myelomonocytic cells differentiated with 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA treatment of human THP-1 cells increased the number of cells that expressed HC...

journal_title：Microbial pathogenesis

authors： Turtinen LW,Seufzer BJ

更新日期：1994-05-01 00:00:00

abstract：:The ability of three C. pneumoniae isolates, Kajaani 6, Helsinki 12 and TW-183, to grow in human umbilical vein endothelial cells (HUVEC) and in an immortalized endothelial cell line EA.hy 926 was studied. All C. pneumoniae isolates were capable of multiplying in endothelial cells. EA.hy926 cells could support the gro...

journal_title：Microbial pathogenesis

authors： Kaukoranta-Tolvanen SS,Laitinen K,Saikku P,Leinonen M

更新日期：1994-04-01 00:00:00

abstract：:The genetic control of mouse susceptibility to Chlamydia psittaci was investigated after intravenous inoculation of 2 x 10(5) plaque-forming units (pfu). Splenic counts 6 days after inoculation gave the level of susceptibility. Results from inoculation of mice from H-2 congenic strains with three different genetic bac...

journal_title：Microbial pathogenesis

authors： Buzoni-Gatel D,Bernard F,Pla M,Rodolakis A,Lantier F

更新日期：1994-03-01 00:00:00

abstract：:Mycobacterium avium complex (MAC) strains from AIDS and non-AIDS patients and from the environment were studied for their colony morphology and virulence in beige mice. The majority of the MAC isolates from AIDS patients, in contrast to those from non-AIDS patients and the environment, showed increased virulence. Simi...

journal_title：Microbial pathogenesis

authors： Reddy VM,Parikh K,Luna-Herrera J,Falkinham JO 3rd,Brown S,Gangadharam PR

更新日期：1994-02-01 00:00:00

abstract：:A variant of Vero toxin 2 (VT2), or Shiga-like toxin II (SLT-II) was purified from a cloned strain of Escherichia coli carrying a gene that encodes SLT-IIva, which is most closely related to SLT-IIv (VT2vp, VTe) in its nucleotide sequence. The purified SLT-IIva showed a slight difference from the purified SLT-IIv (VT2...

journal_title：Microbial pathogenesis

authors： Ohmura M,Cao C,Karasawa T,Okuda J,Kurazono H,Gannon VP,Gyles CL,Takeda Y

更新日期：1993-11-01 00:00:00

abstract：:A DNA fragment from Bordetella pertussis, encoding the fim2 fimbrial subunit gene with adjacent sequences, was used as a probe for the detection of homologous sequences in chromosomal DNA of Bordetella avium. A 1.8 kb Sa1I-PstI fragment from the genome of B. avium, which hybridized with the probe, was isolated and seq...

journal_title：Microbial pathogenesis

authors： Savelkoul PH,de Groot LE,Boersma C,Livey I,Duggleby CJ,van der Zeijst BA,Gaastra W

更新日期：1993-09-01 00:00:00

abstract：:Salmonella spp. have been investigated as live vaccine vectors because they are heat stable and can elicit humoral, cellular, and secretory immune responses. However, the expression of some foreign antigens is toxic to bacterial vectors. We therefore studied an approach for the controlled expression of antigen in Salm...

journal_title：Microbial pathogenesis

authors： Ervin SE,Small PA Jr,Gulig PA

更新日期：1993-08-01 00:00:00

abstract：:Hemolysins are cytolytic proteins which have been extensively characterized at the molecular level, however, their in vivo functions remain unclear. This study analyzed the association of hemolysin production with the inflammatory response in patients with urinary tract infection (UTI). Infants and children with their...

journal_title：Microbial pathogenesis

authors： Connell H,de Man P,Jodal U,Lincoln K,Svanborg C

更新日期：1993-06-01 00:00:00

abstract：:VirB plays a central role in the regulation of virulence of Shigella flexneri. It acts as a transcriptional activator and is itself transcriptionally activated by another virulence protein, VirF. Experiments were performed in order to identify the site upstream of virB at which VirF binds in order to activate transcri...

journal_title：Microbial pathogenesis

authors： Jost BH,Adler B

更新日期：1993-06-01 00:00:00

abstract：:To determine if RecA plays a role in the virulence of Brucella abortus, a B. abortus RecA mutant was constructed and its survival was examined in mice. The recA gene was cloned from a B. abortus genomic DNA library by complementation of an Escherichia coli recA mutant in the presence of methyl methanesulfonate (MMS). ...

journal_title：Microbial pathogenesis

authors： Tatum FM,Morfitt DC,Halling SM

更新日期：1993-03-01 00:00:00

abstract：:The effects of depleting CD4+ and CD8+ T cells on macrophage recruitment have been analyzed for bronchoalveolar lavage (BAL) populations from mice with primary or secondary influenza pneumonia. Macrophages were characterized by both the capacity to engulf latex particles and the expression of mRNA for a 65 kD heat sho...

journal_title：Microbial pathogenesis

authors： Allan W,Carding SR,Eichelberger M,Doherty PC

更新日期：1993-01-01 00:00:00

abstract：:During the course of infection, Yersinia enterocolitica invades tissues where macrophages and polymorphonuclear leucocytes (PMNs) constitute the first line of defence. As expression of virulence in Y. enterocolitica is governed in part by a c. 70 kilobase virulence plasmid (pYV), we investigated the influence of this ...

journal_title：Microbial pathogenesis

authors： Tabrizi SN,Robins-Browne RM

更新日期：1992-09-01 00:00:00

abstract：:A collection of Haemophilus ducreyi isolates were screened for the ability to bind to fibrinogen, fibronectin, collagen, gelatin and laminin by a particle agglutination test using latex beads coated with the individual proteins. Thirteen of 21 isolates reacted with all five extracellular matrix proteins. Binding of or...

journal_title：Microbial pathogenesis

authors： Abeck D,Johnson AP,Mensing H

更新日期：1992-07-01 00:00:00

abstract：:Porphyromonas gingivalis is capable of in vitro growth when iron sources are either complexed to hemin or host iron transport proteins, or exist in an inorganic form. This study examined the effect of these iron sources on outer membrane protein (OMP) expression in P. gingivalis W50. Hemin (iron) starved P. gingivalis...

journal_title：Microbial pathogenesis

authors： Bramanti TE,Holt SC

更新日期：1992-07-01 00:00:00

abstract：:The enteropathogenic potential of 32 Vibrio cholerae O1 isolates that do not produce cholera toxin was examined in the orally inoculated, sealed adult mouse model. Live cultures (2 x 10(10) cfu/ml) of 7/16 clinical and 6/16 environmental isolates produced a positive intestinal fluid accumulation (FA) ratio that reache...

journal_title：Microbial pathogenesis

authors： Moyenuddin M,Wachsmuth K,Richardson SH,Cook WL

更新日期：1992-06-01 00:00:00

abstract：:Bovine leukemia virus (BLV) is transcriptionally silent in most circulating peripheral blood mononuclear cells (PBMCs) of animals with well-established infections. Using PBMCs from a newly infected sheep, we asked whether viral transcription proceeded differently during the initial months of infection, when the preval...

journal_title：Microbial pathogenesis

authors： Radke K,Sigala TJ,Grossman D

更新日期：1992-05-01 00:00:00

abstract：:We have described a new class of live attenuated salmonella vaccines harbouring lesions in htrA, a stress protein gene previously. The virulence and invasiveness of Salmonella htrA mutants was investigated in three models of increased susceptibility to Salmonella infection. These included BALB/c mice, either given sub...

journal_title：Microbial pathogenesis

authors： Strahan K,Chatfield SN,Tite J,Dougan G,Hormaeche CE

更新日期：1992-04-01 00:00:00

abstract：:A total of 36 Escherichia coli urinary tract isolates (UTI) of serotype O6, with different combinations of capsule (K) and flagellin (H) antigens, were analysed according to the outer membrane pattern (OMP), serum resistance properties, mannose-resistant hemagglutination using various types of erythrocytes, and also f...

journal_title：Microbial pathogenesis

authors： Zingler G,Ott M,Blum G,Falkenhagen U,Naumann G,Sokolowska-Köhler W,Hacker J

更新日期：1992-04-01 00:00:00

abstract：:Mouse macrophages activated by gamma interferon (IFN-gamma) and bacterial lipopolysaccharide (LPS) are highly cytotoxic for the enteric protozoan parasite Entamoeba histolytica. Herein, we show that this killing by activated macrophages is L-arginine dependent, inasmuch as it was blocked by exogenous arginase or NG-mo...

journal_title：Microbial pathogenesis

authors： Denis M,Ghadirian E

更新日期：1992-03-01 00:00:00

abstract：:Heat-stable enterotoxins (ST) activate guanylyl cyclase in T84 cells, rapidly and specifically. Activation is monitored by cGMP production and occurs at lower concentrations of ST than required for eliciting fluid accumulation in suckling mice. Atrial natriuretic factor (ANF) neither activates guanylyl cyclase nor mod...

journal_title：Microbial pathogenesis

authors： Visweswariah SS,Shanthi G,Balganesh TS

更新日期：1992-03-01 00:00:00

abstract：:To examine the genetic control of acute and late disease induced by a murine coronavirus, strain JHM (JHMV), BALB/cHeA, STS/A, F1 hybrids and 13 recombinant inbred (RI) strains between BALB/cHeA and STS/A mouse strains were inoculated intracerebrally with 100 pfu of JHMV. All the BALB/cHeA mice died within 2 weeks fro...

journal_title：Microbial pathogenesis

authors： Kyuwa S,Yamaguchi K,Toyoda Y,Fujiwara K,Hilgers J

更新日期：1992-02-01 00:00:00

abstract：:Our previous studies have shown that the adhesive ability of Enterococcus faecalis is dependent on the strain and is further modified by growth in serum. The data reported here demonstrate that E. faecalis adherence is mediated by carbohydrate residues present on the bacterial cell surface. Some of these (D-mannose an...

journal_title：Microbial pathogenesis

authors： Guzmàn CA,Pruzzo C,Platè M,Guardati MC,Calegari L

更新日期：1991-12-01 00:00:00

abstract：:BALB/c mice were infected with 10(5) colony forming units (cfu) of Mycobacterium avium TMC 702 i.v. and the growth of the inoculum followed in the spleens of control mice. Other infected mice given weekly doses of 1 microgram of TGF-b1 or weekly doses of 2 mg of a rabbit antiserum against mouse TGF-b1 were evaluated f...

journal_title：Microbial pathogenesis

authors： Denis M,Ghadirian E

更新日期：1991-11-01 00:00:00

abstract：:The hemA mutation reduces the virulence of Salmonella typhimurium for mice by at least 10(7)-fold, as measured by change in LD50. The hemA mutation does not appear to affect killing of salmonella in mice. The salmonella with the hemA mutation persist in the spleen and liver for 2 to 3 weeks following intravenous injec...

journal_title：Microbial pathogenesis

authors： Benjamin WH Jr,Hall P,Briles DE

更新日期：1991-10-01 00:00:00

abstract：:Phenotypic serum resistance of gonococci in urethral exudates is due to sialylation of lipopolysaccharide (LPS) by host cytidine 5'-monophospho-N-acetyl neuraminic acid (CMP-NANA). A surface structure was visible on gonococci [strain BS4 (agar)] that had been stained with ruthenium red after incubation with CMP-NANA. ...

journal_title：Microbial pathogenesis

authors： Fox AJ,Curry A,Jones DM,Demarco de Hormaeche R,Parsons NJ,Cole JA,Smith H

更新日期：1991-09-01 00:00:00

abstract：:We examined the cells recovered by bronchoalveolar lavage (BAL) during the course of respiratory infection caused by Sendai virus in normal (20% protein diet) and malnourished (2% protein diet) mice. As in our previous experiments, mortality in normal mice was 32% in comparison with 87% in the malnourished group. Viru...

journal_title：Microbial pathogenesis

authors： Lee HJ,Moody CT,Reiss CS,Peña-Cruz V,McIntosh K

更新日期：1991-09-01 00:00:00

abstract：:The extracellular hemolysin (AHH1) gene of Aeromonas hydrophila ATCC7966 was cloned into Charomid9-28 in Escherichia coli DH1, and its complete nucleotide sequence determined. Escherichia coli carrying this gene expressed an extracellular heat-labile hemolysin for rabbit red blood cells. The minimum size of the coding...

journal_title：Microbial pathogenesis

authors： Hirono I,Aoki T

更新日期：1991-09-01 00:00:00

abstract：:The growth inhibitory effects of chicken egg white ovomacroglobulin (ovoM) on Serratia marcescens and Pseudomonas aeruginosa were studied. The growth of protease-producing strains was greater than that of the strains producing little protease, and was inhibited in a dose-dependent manner by ovoM, a potent protease inh...

journal_title：Microbial pathogenesis

authors： Miyagawa S,Nishino N,Kamata R,Okamura R,Maeda H

更新日期：1991-08-01 00:00:00